cPAとは
cyclic
Phosphatidic
Acid
環状ホスファチジン酸（cyclic phosphatidic acid：cPA）は、1985年に当社研究担当（室伏きみ子）が発見した生理活性物質です。
まず、生物学の分野で形態学的・生理学的研究の材料として長年用いられてきた真性粘菌Physarum polycephalumに含まれる新規物質を分離できたことが研究の発端です。その物質に細胞増殖を顕著に抑制する作用が存在することを見出したことで、研究が発展しました。
さらに、cPAは生物に普遍的に含まれる物質でありヒトにも存在すること、そして、
①がんの浸潤・転移抑制作用
②鎮痛作用
③ヒアルロン酸合成促進作用（化粧品への応用実績あり）
④脳や神経に対する作用（神経細胞死抑制作用）
など、多様な効果を有する物質であることを発見しました。
当社では、cPAについての豊富な知見を基に開発を進めています。天然体cPAは、化粧品材料として応用され、既に商品化されています(※)。また様々な誘導体を開発し、それらの医薬応用を目指しています。
(※)当社および室伏きみ子が関与していない企業が製造・販売した化粧品・育毛剤・サプリメントのうち、無断で関与を謳った商品を見つけた際には、記載を即刻削除するよう、引き続き要求して参ります。また、安全性への配慮を欠いた商品を発見いたしました。関係各所（消費者庁など）に連絡し、対応を求めております。
cPAおよび関連論文
Related papers
2023年
2-Carba cyclic phosphatidic acid regulates blood coagulation and fibrinolysis system for repair after brain injury. Endo M, Gotoh M, Nakashima M, Kawamoto Y, Sakai S, Murakami-Murofushi K, Hashimoto K, Miyamoto Y. Brain Res. 2023 Nov 1;1818:148511.
2-carba-cyclic phosphatidic acid modulates astrocyte-to-microglia communication and influences microglial polarization towards an anti-inflammatory phenotype. Takei R, Nakashima M, Gotoh M, Endo M, Hashimoto K, Miyamoto Y, Murakami-Murofushi K. Neurosci Lett. 2023 Feb 16;797:137063.
2021年
The neuroprotective function of 2-carba-cyclic phosphatidic acid: Implications for tenascin-C via astrocytes in traumatic brain injury. Nakashima M, Gotoh M, Hashimoto K, Endo M, Murakami-Murofushi K, Ikeshima-Kataoka H, Miyamoto Y. J Neuroimmunol. 2021 Dec 15;361:577749.
2-Carba-lysophosphatidic acid is a novel β-lysophosphatidic acid analogue with high potential for lysophosphatidic acid receptor activation and autotaxin inhibition. Fukasawa K, Gotoh M, Uwamizu A, Hirokawa T, Ishikawa M, Shimizu Y, Yamamoto S, Iwasa K, Yoshikawa K, Aoki J, Murakami-Murofushi K. Sci Rep. 2021 Aug 30;11(1):17360.
Adenine nucleotide translocase 2, a putative target protein for 2-carba cyclic phosphatidic acid in microglial cells. Tsukahara T, Sahara Y, Ribeiro N, Tsukahara R, Gotoh M, Sakamoto S, Handa H, Murakami-Murofushi K. Cell Signal. 2021 Jun;82:109951.
2020年
Evaluation of the pharmacokinetics of 2-carba-cyclic phosphatidic acid by liquid chromatography-triple quadrupole mass spectrometry. Shimizu Y, Fukasawa K, Yamamoto S, Shibaike Y, Tsukahara R, Ishikawa M, Iwasa K, Yoshikawa K, Gotoh M, Murakami-Murofushi K. Prostaglandins Other Lipid Mediat. 2020 Oct;150:106450.
Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level. Nakajima S, Fukasawa K, Gotoh M, Murakami-Murofushi K, Kunugi H. Int J Obes (Lond). 2020 Mar;44(3):727-738.
2019年
Oleic acid is a potent inducer for lipid droplet accumulation through its esterification to glycerol by diacylglycerol acyltransferase in primary cortical astrocytes. Nakajima S, Gotoh M, Fukasawa K, Murakami-Murofushi K, Kunugi H. Brain Res. 2019 Dec 15;1725:146484.
2-Carba cyclic phosphatidic acid inhibits lipopolysaccharide-induced prostaglandin E2 production in a human macrophage cell line. Shibaike Y, Gotoh M, Ogawa C, Nakajima S, Yoshikawa K, Kobayashi T, Murakami-Murofushi K. Biochem Biophys Rep. 2019 Jul 19;19:100668.
2018年
Qualitative and quantitative comparison of cyclic phosphatidic acid and its related lipid species in rat serum using hydrophilic interaction liquid chromatography with tandem-mass spectrometry. Fukasawa K, Nakajima S, Gotoh M, Tanaka S, Murofushi H, Murakami-Murofushi K. J Chromatogr A. 2018 Sep 14;1567:177-184.
2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex. Hashimoto K, Nakashima M, Hamano A, Gotoh M, Ikeshima-Kataoka H, Murakami-Murofushi K, Miyamoto Y. Sci Rep. 2018 Jun 26;8(1):9715.
Corrigendum to “Quantitative determination of cyclic phosphatidic acid and its carba analog in mouse organs and plasma using LC-MS/MS” [J. Chromatogr. B 1076 (2018) 15-21]. Shimizu Y, Ishikawa M, Gotoh M, Fukasawa K, Yamamoto S, Iwasa K, Yoshikawa K, Murakami-Murofushi K. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jul 15;1090:123-124.
Correction to: Protective and therapeutic role of 2-carbacyclic phosphatidic acid in demyelinating disease. Yamamoto S, Yamashina K, Ishikawa M, Gotoh M, Yagishita S, Iwasa K, Maruyama K, Murakami-Murofushi K, Yoshikawa K. J Neuroinflammation. 2018 Mar 5;15(1):67.
LPA5 signaling is involved in multiple sclerosis-mediated neuropathic pain in the cuprizone mouse model. Tsukahara R, Yamamoto S, Yoshikawa K, Gotoh M, Tsukahara T, Neyama H, Ishii S, Akahoshi N, Yanagida K, Sumida H, Araki M, Araki K, Yamamura KI, Murakami-Murofushi K, Ueda H. J Pharmacol Sci. 2018 Feb;136(2):93-96.
Quantitative determination of cyclic phosphatidic acid and its carba analog in mouse organs and plasma using LC-MS/MS. Shimizu Y, Ishikawa M, Gotoh M, Fukasawa K, Yamamoto S, Iwasa K, Yoshikawa K, Murakami-Murofushi K. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Feb 15;1076:15-21.
2-O-Carba-oleoyl cyclic phosphatidic acid induces glial proliferation through the activation of lysophosphatidic acid receptor. Nakajima S, Gotoh M, Fukasawa K, Murofushi H, Murakami-Murofushi K. Brain Res. 2018 Feb 15;1681:44-51.
Age-related changes in cyclic phosphatidic acid-induced hyaluronic acid synthesis in human fibroblasts. Sano K, Gotoh M, Dodo K, Tajima N, Shimizu Y, Murakami-Murofushi K. Hum Cell. 2018 Jan;31(1):72-77.
2017年
Protective and therapeutic role of 2-carba-cyclic phosphatidic acid in demyelinating disease. Yamamoto S, Yamashina K, Ishikawa M, Gotoh M, Yagishita S, Iwasa K, Maruyama K, Murakami-Murofushi K, Yoshikawa K. J Neuroinflammation. 2017 Jul 21;14(1):142.
2015年
Cyclic phosphatidic acid inhibits the secretion of vascular endothelial growth factor from diabetic human coronary artery endothelial cells through peroxisome proliferator-activated receptor gamma. Tsukahara T, Tsukahara R, Haniu H, Matsuda Y, Murakami-Murofushi K. Mol Cell Endocrinol. 2015 Sep 5;412:320-9.
The effect of cyclic phosphatidic acid on the proliferation and differentiation of mouse cerebellar granule precursor cells during cerebellar development. Konakazawa M, Gotoh M, Murakami-Murofushi K, Hamano A, Miyamoto Y. Brain Res. 2015 Jul 21;1614:28-37.
Ionizing radiation, inflammation, and their interactions in colon carcinogenesis in Mlh1-deficient mice. Morioka T, Miyoshi-Imamura T, Blyth BJ, Kaminishi M, Kokubo T, Nishimura M, Kito S, Tokairin Y, Tani S, Murakami-Murofushi K, Yoshimi N, Shimada Y, Kakinuma S. Cancer Sci. 2015 Mar;106(3):217-26.
2014年
Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh M, Nagano A, Tsukahara R, Murofushi H, Morohoshi T, Otsuka K, Murakami-Murofushi K. Mol Pain. 2014 Aug 14;10:52.
Cyclic phosphatidic acid treatment suppreses cuprizone-induced demyelination and motor dysfunction in mice. Yamamoto S, Gotoh M, Kawamura Y, Yamashina K, Yagishita S, Awaji T, Tanaka M, Maruyama K, Murakami-Murofushi K, Yoshikawa K. Eur J Pharmacol. 2014 Oct 15;741:17-24.
Cyclic phosphatidic acid and lysophosphatidic acid induce hyaluronic acid synthesis via CREB transcription factor regulation in human skin fibroblasts. Maeda-Sano K, Gotoh M, Morohoshi T, Someya T, Murofushi H, Murakami-Murofushi K. Biochim Biophys Acta. 2014 Sep;1841(9):1256-63.
2013年
Cholesterol glucosylation is catalyzed by transglucosylation reaction of β-glucosidase 1. Akiyama H, Kobayashi S, Hirabayashi Y, Murakami-Murofushi K. Biochem Biophys Res Commun. 2013 Nov 29;441(4):838-43.
The macroscopic structure of RADA16 peptide hydrogel stimulates monocyte/macrophage differentiation in HL60 cells via cholesterol synthesis. Kakiuchi Y, Hirohashi N, Murakami-Murofushi K. Biochem Biophys Res Commun. 2013 Apr 12;433(3):298-304.
2012年
Protection of neuroblastoma Neuro2A cells from hypoxia-induced apoptosis by cyclic phosphatidic acid (cPA). Gotoh M, Sano-Maeda K, Murofushi H, Murakami-Murofushi K. PLoS One. 2012;7(12):e51093.
Release of cyclic phosphatidic acid from gelatin-based hydrogels inhibit colon cancer cell growth and migration. Tsukahara T, Murakami-Murofushi K. Sci Rep. 2012;2:687.
Pharmacological evaluation of a novel cyclic phosphatidic acid derivative 3-S-cyclic phosphatidic acid (3-S-cPA). Nozaki E, Gotoh M, Tanaka R, Kato M, Suzuki T, Nakazaki A, Hotta H, Kobayashi S, Murakami-Murofushi K. Bioorg Med Chem. 2012 May 15;20(10):3196-201.
Controlling cancer through the autotaxin-lysophosphatidic acid receptor axis. Gotoh M, Fujiwara Y, Yue J, Liu J, Lee S, Fells J, Uchiyama A, Murakami-Murofushi K, Kennel S, Wall J, Patil R, Gupte R, Balazs L, Miller DD, Tigyi G. J Biochem Soc Trans. 2012 Feb;40(1):31-6.
2011年
Efficient synthesis of 3-O-thia-cPA and preliminary analysis of its biological activity toward autotaxin. Tanaka R, Kato M, Suzuki T, Nakazaki A, Nozaki E, Gotoh M, Murakami-Murofushi K, Kobayashi S. Bioorg Med Chem Lett. 2011 Jul 15;21(14):4180-2.
Antinociceptive effect of cyclic phosphatidic acid and its derivative on animal models of acute and chronic pain. Kakiuchi Y, Nagai J, Gotoh M, Hotta H, Murofushi H, Ogawa T, Ueda H, Murakami-Murofushi K. Mol Pain. 2011 May 14;7:33.
DNA packaging proteins Glom and Glom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum. Itoh K, Izumi A, Mori T, Dohmae N, Yui R, Maeda-Sano K, Shirai Y, Kanaoka MM, Kuroiwa T, Higashiyama T, Sugita M, Murakami-Murofushi K, Kawano S, Sasaki N. Mitochondrion. 2011 Jul;11(4):575-86.
Novel sterol glucosyltransferase in the animal tissue and cultured cells: evidence that glucosylceramide as glucose donor. Akiyama H, Sasaki N, Hanazawa S, Gotoh M, Kobayashi S, Hirabayashi Y, Murakami-Murofushi K. Biochim Biophys Acta. 2011 May;1811(5):314-22.
Synthesis of enantiopure 2-carba-cyclic phosphatidic acid and effects of its chirality on biological functions. Nozaki E, Gotoh M, Hotta H, Hanazawa S, Kobayashi S, Murakami-Murofushi K. Biochim Biophys Acta. 2011 Apr;1811(4):271-7.
Cyclic phosphatidic acid influences the expression and regulation of cyclic nucleotide phosphodiesterase 3B and lipolysis in 3T3-L1 cells. Tsukahara T, Hanazawa S, Murakami-Murofushi K. Biochem Biophys Res Commun. 2011 Jan 7;404(1):109-14.
2010年
Synthesis and pharmacological evaluation of the stereoisomers of 3-carba cyclic-phosphatidic acid. Gupte R, Siddam A, Lu Y, Li W, Fujiwara Y, Panupinthu N, Pham TC, Baker DL, Parrill AL, Gotoh M, Murakami-Murofushi K, Kobayashi S, Mills GB, Tigyi G, Miller DD. Bioorg Med Chem Lett. 2010 Dec 15;20(24):7525-8.
Cyclic phosphatidic acid decreases proliferation and survival of colon cancer cells by inhibiting peroxisome proliferator-activated receptor γ. Tsukahara T, Hanazawa S, Kobayashi T, Iwamoto Y, Murakami-Murofushi K. Prostaglandins Other Lipid Mediat. 2010 Nov;93(3-4):126-33.
Effects of cyclic phosphatidic acid on delayed neuronal death following transient ischemia in rat hippocampal CA1. Gotoh M, Hotta H, Murakami-Murofushi K. Eur J Pharmacol. 2010 Dec 15;649(1-3):206-9.
Effect of endurance exercise training on oxidative stress in spontaneously hypertensive rats (SHR) after emergence of hypertension. Kimura H, Kon N, Furukawa S, Mukaida M, Yamakura F, Matsumoto K, Sone H, Murakami-Murofushi K. Clin Exp Hypertens. 2010;32(7):407-15.
Lysophosphatidic acid-activated Cl- current activity in human systemic sclerosis skin fibroblasts. Yin Z, Carbone LD, Gotoh M, Postlethwaite A, Bolen AL, Tigyi GJ, Murakami-Murofushi K, Watsky MA. Rheumatology (Oxford). 2010 Dec;49(12):2290-7.
Phospholipase D2-dependent inhibition of the nuclear hormone receptor PPARgamma by cyclic phosphatidic acid. Tsukahara T, Tsukahara R, Fujiwara Y, Yue J, Cheng Y, Guo H, Bolen A, Zhang C, Balazs L, Re F, Du G, Frohman MA, Baker DL, Parrill AL, Uchiyama A, Kobayashi T, Murakami-Murofushi K, Tigyi G. Mol Cell. 2010 Aug 13;39(3):421-32.
(S)-FTY720-vinylphosphonate, an analogue of the immunosuppressive agent FTY720, is a pan-antagonist of sphingosine 1-phosphate GPCR signaling and inhibits autotaxin activity. Valentine WJ, Kiss GN, Liu J, E S, Gotoh M, Murakami-Murofushi K, Pham TC, Baker DL, Parrill AL, Lu X, Sun C, Bittman R, Pyne NJ, Tigyi G. Cell Signal. 2010 Oct;22(10):1543-53.
Unique characteristics of radiation-induced apoptosis in the postnatally developing small intestine and colon of mice. Miyoshi-Imamura T, Kakinuma S, Kaminishi M, Okamoto M, Takabatake T, Nishimura Y, Imaoka T, Nishimura M, Murakami-Murofushi K, Shimada Y. Radiat Res. 2010 Mar;173(3):310-8.
2009年
MOV10 as a novel telomerase-associated protein. Nakano M, Kakiuchi Y, Shimada Y, Ohyama M, Ogiwara Y, Sasaki-Higashiyama N, Yano N, Ikeda F, Yamada E, Iwamatsu A, Kobayashi K, Nishiyama K, Ichikawa S, Kaji K, Ide T, Murofushi H, Murakami-Murofushi K. Biochem Biophys Res Commun. 2009 Oct 16;388(2):328-32.
The Plasmodium HU homolog, which binds the plastid DNA sequence-independent manner, is essential for the parasite’s survival. Sasaki N, Hirai M, Maeda K, Yui R, Itoh K, Namiki S, Morita T, Hata M, Murakami-Murofushi K, Matsuoka H, Kita K, Sato S. FEBS Lett. 2009 May 6;583(9):1446-50.
2008年
Early weaning induces anxiety and precocious myelination in the anterior part of the basolateral amygdala of male Balb/c mice. Ono M, Kikusui T, Sasaki N, Ichikawa M, Mori Y, Murakami-Murofushi K. Neuroscience. 2008 Oct 28;156(4):1103-10.
2007年
Pertuzumab, a novel HER dimerization inhibitor, inhibits the growth of human lung cancer cells mediated by the HER3 signaling pathway. Sakai K, Yokote H, Murakami-Murofushi K, Tamura T, Saijo N, Nishio K. Cancer Sci. 2007 Sep;98(9):1498-503.
STAT3 and PKC differentially regulate telomerase activity during megakaryocytic differentiation of K562 cells. Nakatake M, Kakiuchi Y, Sasaki N, Murakami-Murofushi K, Yamada O. Cell Cycle. 2007 Jun 15;6(12):1496-501.
Inhibition of transcellular tumor cell migration and metastasis by novel carba-derivatives of cyclic phosphatidic acid. Uchiyama A, Mukai M, Fujiwara Y, Kobayashi S, Kawai N, Murofushi H, Inoue M, Enoki S, Tanaka Y, Niki T, Kobayashi T, Tigyi G, Murakami-Murofushi K. Biochim Biophys Acta. 2007 Jan;1771(1):103-12.
2006年
Cyclic phosphatidic acid is produced by autotaxin in blood. Tsuda S, Okudaira S, Moriya-Ito K, Shimamoto C, Tanaka M, Aoki J, Arai H, Murakami-Murofushi K, Kobayashi T. J Biol Chem. 2006 Sep 8;281(36):26081-8.
Cyclic phosphatidic acid stimulates respiration without producing vasopressor or tachycardiac effects in rats. Hotta H, Kagitani F, Murakami-Murofushi K. Eur J Pharmacol. 2006 Aug 14;543(1-3):27-31.
Carba analogs of cyclic phosphatidic acid are selective inhibitors of autotaxin and cancer cell invasion and metastasis. Baker DL, Fujiwara Y, Pigg KR, Tsukahara R, Kobayashi S, Murofushi H, Uchiyama A, Murakami-Murofushi K, Koh E, Bandle RW, Byun HS, Bittman R, Fan D, Murph M, Mills GB, Tigyi G. J Biol Chem. 2006 Aug 11;281(32):22786-93.
In-frame deletion in the EGF receptor alters kinase inhibition by gefitinib. Sakai K, Yokote H, Murakami-Murofushi K, Tamura T, Saijo N, Nishio K. Biochem J. 2006 Aug 1;397(3):537-43.
Regulation of levels of actin threonine phosphorylation during life cycle of Physarum polycephalum. Shirai Y, Sasaki N, Kishi Y, Izumi A, Itoh K, Sameshima M, Kobayashi T, Murakami-Murofushi K. Cell Motil Cytoskeleton. 2006 Feb;63(2):77-87.
2005年
Identification of residues responsible for ligand recognition and regioisomeric selectivity of lysophosphatidic acid receptors expressed in mammalian cells. Fujiwara Y, Sardar V, Tokumura A, Baker D, Murakami-Murofushi K, Parrill A, Tigyi G. J Biol Chem. 2005 Oct 14;280(41):35038-50.
2004年
A novel pyrazolone, 4,4-dichloro-1-(2,4-dichlorophenyl)-3-methyl-5-pyrazolone, as a potent catalytic inhibitor of human telomerase. Kakiuchi Y, Sasaki N, Satoh-Masuoka M, Murofushi H, Murakami-Murofushi K. Biochem Biophys Res Commun. 2004 Aug 6;320(4):1351-1358.
Attenuation by cyclic phosphatidic acid of peritoneal metastasis of azoxymethane-induced intestinal cancers in Wistar rats. Ishihara R, Tatsuta M, Iishi H, Baba M, Uedo N, Higashino K, Mukai M, Ishiguro S, Kobayashi S, Murakami-Murofushi K. Int J Cancer. 2004 Jun 10;110(2):188-93.
Transient posttranslational up-regulation of telomerase activity during megakaryocytic differentiation of K562 cells. Nakatake M, Sasaki N, Murakami-Murofushi K, Yamada O. Biochem Biophys Res Commun. 2004 Feb 20;314(4):1080-5.
2003年
Cyclic phosphatidic acid elicits neurotrophin-like actions in embryonic hippocampal neurons. Fujiwara Y, Sebök A, Meakin S, Kobayashi T, Murakami-Murofushi K, Tigyi G. J Neurochem. 2003 Dec;87(5):1272-83.
Glom is a novel mitochondrial DNA packaging protein in Physarum polycephalum and causes intense chromatin condensation without suppressing DNA functions. Sasaki N, Kuroiwa H, Nishitani C, Takano H, Higashiyama T, Kobayashi T, Shirai Y, Sakai A, Kawano S, Murakami-Murofushi K, Kuroiwa T. Mol Biol Cell. 2003 Dec;14(12):4758-69.
Cholesteryl glucoside-induced protection against gastric ulcer. Kunimoto S, Murofushi W, Yamatsu I, Hasegawa Y, Sasaki N, Kobayashi S, Kobayashi T, Murofushi H, Murakami-Murofushi K. Cell Struct Funct. 2003 Jun;28(3):179-86.
Cyclic phosphatidic acid inhibits RhoA-mediated autophosphorylation of FAK at Tyr-397 and subsequent tumor-cell invasion. Mukai M, Iwasaki T, Tatsuta M, Togawa A, Nakamura H, Murakami-Murofushi K, Kobayashi S, Imamura F, Inoue M. Int J Oncol. 2003 Jun;22(6):1247-56.
Structural and enzymatic characterization of physarolisin (formerly physaropepsin) proves that it is a unique serine-carboxyl proteinase. Nishii W, Ueki T, Miyashita R, Kojima M, Kim YT, Sasaki N, Murakami-Murofushi K, Takahashi K. Biochem Biophys Res Commun. 2003 Feb 21;301(4):1023-9.
2002年
Steryl glucoside is a lipid mediator in stress-responsive signal transduction. Kunimoto S, Murofushi W, Kai H, Ishida Y, Uchiyama A, Kobayashi T, Kobayashi S, Murofushi H, Murakami-Murofushi K. Cell Struct Funct. 2002 Jun;27(3):157-62.
A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae. Kato U, Emoto K, Fredriksson C, Nakamura H, Ohta A, Kobayashi T, Murakami-Murofushi K, Kobayashi T, Umeda M. J Biol Chem. 2002 Oct 4;277(40):37855-62.
Biological functions of a novel lipid mediator, cyclic phosphatidic acid. Murakami-Murofushi K, Uchiyama A, Fujiwara Y, Kobayashi T, Kobayashi S, Mukai M, Murofushi H, Tigyi G. Biochim Biophys Acta. 2002 May 23;1582(1-3):1-7.
2001年
Bioactions and synthesis of a novel lipid mediator, cyclic phosphatidic acid. Kobayashi T, Murakami-Murofushi K. World Rev Nutr Diet. 2001;88:228-32.
Altered expression of HES-1, BETA2/NeuroD, and PDX-1 is involved in impaired insulin synthesis induced by glucocorticoids in HIT-T15 cells. Shinozuka Y, Okada M, Oki T, Sagane K, Mizui Y, Tanaka I, Katayama K, Murakami-Murofushi K. Biochem Biophys Res Commun. 2001 Sep 14;287(1):229-35.
2000年
A novel lipid mediator, cyclic phosphatidic acid (cPA), and its biological functions. Murakami-Murofushi K, Mukai M, Kobayashi S, Kobayashi T, Tigyi G, Murofushi H. Ann N Y Acad Sci. 2000 Apr;905:319-21.
Characterization of endogenous and heterologously expressed LPA receptor subtypes. Fischer DJ, Murakami-Murofushi K, Tigyi G. Ann N Y Acad Sci. 2000 Apr;905:287-9.
Expression of cholesteryl glucoside by heat shock in human fibroblasts. Kunimoto S, Kobayashi T, Kobayashi S, Murakami-Murofushi K. Cell Stress Chaperones. 2000 Jan;5(1):3-7.
1999年
Determinants of receptor subtype specificity in the LPA-like lipid mediator family. Tigyi G, Fisher DJ, Lilion K, Guo Z, Virag T, Sun G, Miller DD, Murakami-Murofushi K, Kobayashi S, Erickson JR. Adv Exp Med Biol. 1999;469:245-51.
Existence of a bioactive lipid, cyclic phosphatidic acid, bound to human serum albumin. Kobayashi T, Tanaka-Ishii R, Taguchi R, Ikezawa H, Murakami-Murofushi K. Life Sci. 1999;65(21):2185-91.
Molecular cloning and characterization of a novel human G-protein-coupled receptor, EDG7, for lysophosphatidic acid. Bandoh K, Aoki J, Hosono H, Kobayashi S, Kobayashi T, Murakami-Murofushi K, Tsujimoto M, Arai H, Inoue K. J Biol Chem. 1999 Sep 24;274(39):27776-85.
Hyperosmotic stress-induced reorganization of actin bundles in Dictyostelium cells over-expressing cofilin. Aizawa H, Katadae M, Maruya M, Sameshima M, Murakami-Murofushi K, Yahara I. Genes Cells. 1999 Jun;4(6):311-24.
Occurrence, metabolism and bioactions of lysophosphatidic acid and cyclic phosphatidic acid as lysophospholipid mediators. Kobayashi T, Murakami-Murofushi K. Tanpakushitsu Kakusan Koso. 1999 Jun;44(8 Suppl):1118-25.
Function of sterylglucoside in cell differentiation and stress responses. Murakami-Murofushi K, Kobayashi T. Tanpakushitsu Kakusan Koso. 1999 Jun;44(8 Suppl):1111-7.
Inhibition of tumor invasion and metastasis by a novel lysophosphatidic acid (cyclic LPA). Mukai M, Imamura F, Ayaki M, Shinkai K, Iwasaki T, Murakami-Murofushi K, Murofushi H, Kobayashi S, Yamamoto T, Nakamura H, Akedo H. Int J Cancer. 1999 Jun 11;81(6):918-22.
1998年
Naturally occurring analogs of lysophosphatidic acid elicit different cellular responses through selective activation of multiple receptor subtypes. Fischer DJ, Liliom K, Guo Z, Nusser N, Virág T, Murakami-Murofushi K, Kobayashi S, Erickson JR, Sun G, Miller DD, Tigyi G. Mol Pharmacol. 1998 Dec;54(6):979-88.
Distinct roles of two separable in vitro activities of yeast Mre11 in mitotic and meiotic recombination. Furuse M, Nagase Y, Tsubouchi H, Murakami-Murofushi K, Shibata T, Ohta K. EMBO J. 1998 Nov 2;17(21):6412-25.
A novel 66-kDa stress protein, p66, associated with the process of cyst formation of Physarum polycephalum is a Physarum homologue of a yeast actin-interacting protein, AIP1. Matsumoto S, Ogawa M, Kasakura T, Shimada Y, Mitsui M, Maruya M, Isohata M, Yahara I, Murakami-Murofushi K. J Biochem. 1998 Aug;124(2):326-31.
1997年
Cell cycle-dependent activation of telomerase in naturally synchronized culture of a true slime mold, Physarum polycephalum. Shimada Y, Nakano M, Kanda N, Murakami-Murofushi K, Kim JK, Ide T, Murofushi H. Biochem Biophys Res Commun. 1997 Mar 17;232(2):492-6.
Heat stress induces a glycosylation of membrane sterol in myxoamoebae of a true slime mold, Physarum polycephalum. Murakami-Murofushi K, Nishikawa K, Hirakawa E, Murofushi H. J Biol Chem. 1997 Jan 3;272(1):486-9.
1996年
Activation of a Ca(2+)-dependent protein kinase in response in heat shock in the myxoamoebae of a true slime mold, Physarum polycephalum. Maruya M, Mitsui M, Murakami-Murofushi K. Cell Struct Funct. 1996 Dec;21(6):533-8.
Xenopus oocytes express multiple receptors for LPA-like lipid mediators. Liliom K, Murakami-Murofushi K, Kobayashi S, Murofushi H, Tigyi G. Am J Physiol. 1996 Mar;270(3 Pt 1):C772-7.
1995年
Reduced fidelity of DNA synthesis in cell extracts from chemically induced primary thymic lymphomas of mice. Kubota Y, Murakami-Murofushi K, Shimada Y, Ogiu T, Oikawa T. Cancer Res. 1995 Sep 1;55(17):3777-80.
Selective inhibition of DNA polymerase-alpha family with chemically synthesized derivatives of PHYLPA, a unique Physarum lysophosphatidic acid. Murakami-Murofushi K, Kobayashi S, Onimura K, Matsumoto M, Shioda M, Yoshida S, Shoji M, Murofushi H. Biochim Biophys Acta. 1995 Aug 24;1258(1):57-60.
Side-chain structure is critical for the transport of sterols from lysosomes to cytoplasm. Sato Y, Nishikawa K, Aikawa K, Mimura K, Murakami-Murofushi K, Arai H, Inoue K. Biochim Biophys Acta. 1995 Jun 27;1257(1):38-46.
Chinese hamster ovary cells expressing a novel type of acetylated low density lipoprotein receptor. Isolation and characterization. Fukasawa M, Hirota K, Adachi H, Mimura K, Murakami-Murofushi K, Tsujimoto M, Arai H, Inoue K. J Biol Chem. 1995 Jan 27;270(4):1921-7.
A novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum. Murakami-Murofushi K, Takahashi T, Murofushi H, Takahashi K. Adv Exp Med Biol. 1995;362:565-8.
1993年
Modulation of DNA synthesis by microtubule-associated protein 2 in the nuclear matrix isolated from Physarum polycephalum. Shioda M, Okuhara K, Murakami-Murofushi K, Murofushi H. Biochem Mol Biol Int. 1993 Dec;31(5):905-10.
Inhibition of cell proliferation by a unique lysophosphatidic acid, PHYLPA, isolated from Physarum polycephalum: signaling events of antiproliferative action by PHYLPA. Murakami-Murofushi K, Kaji K, Kano K, Fukuda M, Shioda M, Murofushi H. Cell Struct Funct. 1993 Oct;18(5):363-70.
Isolation of a new species of Physarum lysophosphatidic acid, PHYLPA, and its effect on DNA polymerase activity. Takahashi Y, Shimada Y, Shioda M, Yoshida S, Murofushi H, Murakami-Murofushi K. Cell Struct Funct. 1993 Jun;18(3):135-8.
Initiation of DNA synthesis in a high molecular weight fraction of Xenopus egg extract. Okuhara K, Shioda M, Shiokawa K, Murakami-Murofushi K, Seyama Y, Murofushi H. Cell Biol Int. 1993 Mar;17(3):275-82. doi: 10.1006/cbir.1993.1063.
1992年
Inhibition of eukaryotic DNA polymerase alpha with a novel lysophosphatidic acid (PHYLPA) isolated from myxoamoebae of Physarum polycephalum. Murakami-Murofushi K, Shioda M, Kaji K, Yoshida S, Murofushi H. J Biol Chem. 1992 Oct 25;267(30):21512-7.
Expression of a 66-kD heat shock protein associated with the process of cyst formation of a true slime mold, Physarum polycephalum. Shimada Y, Kasakura T, Yokota M, Miyata Y, Murofushi H, Sakai H, Yahara I, Murakami-Murofushi K. Cell Struct Funct. 1992 Oct;17(5):301-9.
1991年
Stimulation of DNA polymerase alpha activity by microtubule-associated proteins. Shioda M, Okuhara K, Murofushi H, Mori A, Sakai H, Murakami-Murofushi K, Suzuki M, Yoshida S. Biochemistry. 1991 Dec 3;30(48):11403-12.
1990年
Purification and characterization of a novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum. Murakami-Murofushi K, Takahashi T, Minowa Y, Iino S, Takeuchi T, Kitagaki-Ogawa H, Murofushi H, Takahashi K. J Biol Chem. 1990 Nov 15;265(32):19898-903.
Changes in phospholipid composition and phospholipase D activity during the differentiation of Physarum polycephalum. Minowa A, Kobayashi T, Shimada Y, Maeda H, Murakami-Murofushi K, Ohta J, Inoue K. Biochim Biophys Acta. 1990 Apr 2;1043(2):129-33. doi: 10.1016/0005-2760(90)90285-6.
1989年
Expression of UDP-glucose: poriferasterol glucosyltransferase in the process of differentiation of a true slime mold, Physarum polycephalum. Murakami-Murofushi K, Ohta J. Biochim Biophys Acta. 1989 Sep 15;992(3):412-5.
DNA synthesis by the isolated nuclear matrix from synchronized plasmodia of Physarum polycephalum. Shioda M, Matsuzawa Y, Murakami-Murofushi K, Ohta J. Biochim Biophys Acta. 1989 Apr 12;1007(3):254-63.
Microtubule-associated protein-2 stimulates DNA synthesis catalyzed by the nuclear matrix. Shioda M, Murofushi H, Murakami-Murofushi K, Sakai H. Biochem Biophys Res Commun. 1989 Mar 15;159(2):834-41.
Changes in activity and subcellular localization of alpha-like DNA polymerase during cell cycle of Physarum polycephalum. Shioda M, Murakami-Murofushi K. Biochem Int. 1989 Mar;18(3):581-8.
1987年
Expression of poriferasterol monoglucoside associated with differentiation of Physarum polycephalum. Murakami-Murofushi K, Nakamura K, Ohta J, Suzuki M, Suzuki A, Murofushi H, Yokota T. J Biol Chem. 1987 Dec 5;262(34):16719-23.
Selective inhibition of DNA polymerase alpha by a polysaccharide purified from slime of Physarum polycephalum. Shioda M, Murakami-Murofushi K. Biochem Biophys Res Commun. 1987 Jul 15;146(1):61-6.
DNA synthesis in isolated nuclei from synchronized plasmodia of Physarum polycephalum. Matsuzawa Y, Murakami-Murofushi K, Ohta J, Shioda M. Cell Struct Funct. 1987 Jun;12(3):243-50.
1985年
Visualization method for sulfolipids and its application to the determination of nanomole quantities of lipid sulfur. Murakami-Murofushi K, Nakamura K, Ishizuka I, Ohta J. Anal Biochem. 1985 Sep;149(2):480-3. doi: 10.1016/0003-2697(85)90602-5.
1983年
A novel trisialosyl ganglioside, IV3 alpha (NeuAc)3nLcOse4Cer, from hog kidney cortex. Murakami-Murofushi K, Tadano K, Ishizuka I. J Biochem. 1983 Feb;93(2):621-9.
1982年
Isolation of argininosuccinase from bovine brain: catalytic, physical and chemical properties compared to liver and kidney enzymes. Murakami-Murofushi K, Ratner S. Adv Exp Med Biol. 1982;153:277-89.
1981年
A trisialosyl ganglioside GT3 of hog kidney. Structure and biosynthesis in vitro. Murakami-Murofushi K, Tadano K, Koyama I, Ishizuka I. J Biochem. 1981 Dec;90(6):1817-20.
1980年
A new radiochemical assay for argininosuccinase with purified [14C]argininosuccinate. Ratner S, Murakami-Murofushi K. Anal Biochem. 1980 Jul 15;106(1):134-47.
1979年
Argininosuccinase from bovine brain: isolation and comparison of catalytic, physical, and chemical properties with the enzymes from liver and kidney. Murakami-Murofushi K, Ratner S. Anal Biochem. 1979 May;95(1):139-55.
1977年
Stimulation of sea urchin DNA polymerase by protein factors. II. Formation of active complex in DNA polymerase reaction. Murakami-Murofushi K, Mano Y. Biochim Biophys Acta. 1977 Mar 18;475(2):254-66.
1976年
A cytoplasmic inhibitor of DNA polymerase from the plasmodia of Physarum polycephalum. Murakami-Murofushi K, Nagano H, Mano Y. J Biochem. 1976 Oct;80(4):735-41.
